Journal: Respiratory Research

Article Title: Interleukin-17A mRNA and protein expression within cells from the human bronchoalveolar space after exposure to organic dust

doi: 10.1186/1465-9921-6-44

Figure Lengend Snippet: Messenger RNA for IL-17A in BAL cells . Messenger RNA for IL-17A in BAL cells (percentage of the house-keeping gene HPRT), measured 2 weeks before and 24 hours after exposure to organic dust in a swine confinement. Data are shown as individual (rhombs) plus median (bold horizontal lines) values. *: p < 0,0167; n = 6

Article Snippet: The intracellular expression of IL-17A protein was assessed utilising cytospin slides incubated with a polyclonal goat anti-human IL-17A antibody (R&D: 1 hr).

Techniques:

Journal: Respiratory Research

Article Title: Interleukin-17A mRNA and protein expression within cells from the human bronchoalveolar space after exposure to organic dust

doi: 10.1186/1465-9921-6-44

Figure Lengend Snippet: Immunocytochemical detection of IL-17A protein in BAL lymphocytes . BAL lymphocytes expressing IL-17A IR (brown arrows) after exposure to organic dust in a swine confinement, detected using immunocytochemistry.

Article Snippet: The intracellular expression of IL-17A protein was assessed utilising cytospin slides incubated with a polyclonal goat anti-human IL-17A antibody (R&D: 1 hr).

Techniques: Expressing, Immunocytochemistry

Journal: Respiratory Research

Article Title: Interleukin-17A mRNA and protein expression within cells from the human bronchoalveolar space after exposure to organic dust

doi: 10.1186/1465-9921-6-44

Figure Lengend Snippet: BAL lymphocytes expressing IL-17A protein . Percentage of BAL lymphocytes expressing IL-17A IR before and after the exposure to organic dust in a swine confinement. Data are shown as individual (rhombs) plus median (bold horizontal lines) values. NS: p > 0,0167; n = 6

Article Snippet: The intracellular expression of IL-17A protein was assessed utilising cytospin slides incubated with a polyclonal goat anti-human IL-17A antibody (R&D: 1 hr).

Techniques: Expressing

Journal:

Article Title: Confluent Monolayers of Cultured Human Fetal Retinal Pigment Epithelium Exhibit Morphology and Physiology of Native Tissue

doi: 10.1167/iovs.05-1622

Figure Lengend Snippet: Polarized secretion of VEGF and PEDF in cultured human fetal RPE. Media from apical and basal baths were collected separately at 8 weeks after the cells were seeded onto cell culture inserts. ELISA for VEGF and PEDF were performed in triplicate for each of the 12 wells (24 samples). VEGF protein in the basal bath was 1.7-fold higher than in the apical bath (n = 9; P < 0.005), whereas PEDF protein in the apical bath was 2.3-fold higher than in the basal bath (n = 9; P < 0.05).

Article Snippet: A 96-well EIA plate (Corning Costar) was coated with 100 μL/well of 0.4 μg/mL polyclonal goat anti-human VEGF antibody (R&D Systems, Minneapolis, MN) overnight in PBS (pH 7.4).

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

Journal:

Article Title: The Role of Ultraviolet Irradiation and Heparin-Binding Epidermal Growth Factor-Like Growth Factor in the Pathogenesis of Pterygium

doi:

Figure Lengend Snippet: Expression of HB-EGF in pterygia. Pterygium tissue (A through F), normal limbus (G), and normal conjuntiva (H) were analyzed by immunohistochemistry to determine the expression of HB-EGF. Sections A, C, E, G, and H included the anti-HB-EGF antibody and sections B, D, and F were incubated without primary antibody and served as controls. All tissue sections were counterstained with hematoxylin. These data are representative of all pterygia examined. Original magnifications, ×600.

Article Snippet: 22 After blocking specimens in swine serum, a goat anti-human HB-EGF antibody (R&D Systems) was added to each section overnight at 4°C.

Techniques: Expressing, Immunohistochemistry, Incubation

Journal:

Article Title: The Role of Ultraviolet Irradiation and Heparin-Binding Epidermal Growth Factor-Like Growth Factor in the Pathogenesis of Pterygium

doi:

Figure Lengend Snippet: Genes Up-Regulated in PECs 12 Hours after UVB Exposure Compared to Nonirradiated PECs

Article Snippet: 22 After blocking specimens in swine serum, a goat anti-human HB-EGF antibody (R&D Systems) was added to each section overnight at 4°C.

Techniques:

Journal:

Article Title: The Role of Ultraviolet Irradiation and Heparin-Binding Epidermal Growth Factor-Like Growth Factor in the Pathogenesis of Pterygium

doi:

Figure Lengend Snippet: RT-PCR analysis for HB-EGF (750 bp) mRNA in UVB stimulated PEC (A) and LEC (C). B and D represent RT-PCR for GAPDH (240 bp) in PEC and LEC, respectively. Equal amounts of total RNA were reverse-transcribed from both cell types in all lanes. Time points at 0, 2, 6, 12, and 24 hours after irradiation are represented by lanes 2 to 6, respectively. When no reverse-transcriptase enzyme and no gene-specific primers were included, no PCR product formed (lanes 7 and 8, respectively). A 100-bp ladder (Promega) was run in parallel (lane 1). These results are representative of triplicate experiments in three different cell lines.

Article Snippet: 22 After blocking specimens in swine serum, a goat anti-human HB-EGF antibody (R&D Systems) was added to each section overnight at 4°C.

Techniques: Reverse Transcription Polymerase Chain Reaction, Reverse Transcription, Irradiation

Journal:

Article Title: The Role of Ultraviolet Irradiation and Heparin-Binding Epidermal Growth Factor-Like Growth Factor in the Pathogenesis of Pterygium

doi:

Figure Lengend Snippet: Genes Up-Regulated in PECs 6 Hours after UVB Exposure Compared to Nonirradiated PECs

Article Snippet: 22 After blocking specimens in swine serum, a goat anti-human HB-EGF antibody (R&D Systems) was added to each section overnight at 4°C.

Techniques: